Using RAPD Markers

AIWKKY. Thirty-four extant pawpaw [&imina w&& (L.) Dunsl] cultivars and advancedselections representing a large portion of the gene pool of cultivated pawpaws were investigated using71 randomly amplified polymorphic DNA (RAPD) markers to es tabl i sh genet ic ident i t ies and evaluate genet ic re latedness . All 34 cu l t ivated pawpaws were uniquely identified by as few as 14 loci of eight primers. Genetic diversity of the existing gene pool of cultivated pawpaws, as estimated by Nei’s gene diversity (He), was similar to that of wild pawpaw populations. The genetic relatedness among the cultivated pawpaws examined by UPGMA chaster analysis separated 34 cultivars and selections into two distinct clusters, a cluster of PPF (The PawPaw Foundation) selections and a clwter including a majority of the extant cultivars selected from the wild and their derived selections. The results are in general agreement with the known selection history and pedigree information available. The consensus fingerprint profile using the genetically defined RAPD markers is a useful and reliable method for establishing the genetic identities of the pawpaw cultivars and advanced selections. This also proved to be an improved discriminating tool over isozyme markers for the assessment of genetic diversity and relatedness. RAPD profiling of data presented in this study provides a useful reference for germplasm curators engaged in making decisions of sampling strategies, germulasm management and for breeders deciding which parents to select for future breeding efforts.’ .-